Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator
نویسندگان
چکیده
Abstract Menaquinone-7 (MK-7) is a kind of vitamin K2 playing an important role in the treatment and prevention cardiovascular disease, osteoporosis arterial calcification. The purpose this study to establish adaptive evolution strategy based on chemical modulator improve MK-7 biosynthesis Bacillus natto . inhibitor 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase), glyphosate, was chosen as perform experiments. final strain ALE-25–40, which obtained after 40 cycles 25 mmol/L showed maximal titer 62 mg/L productivity 0.42 mg/(L h), representing 2.5 3 times original strain, respectively. Moreover, ALE-25–40 generated fewer spores higher NADH redox potential. Furthermore, mechanism related improved performance investigated by comparative transcriptomics analysis. Genes sporation formation were down-regulated. In addition, several genes also up-regulated. This proposed here may provide new alternative directive for industrial production K2. Graphical
منابع مشابه
Advances in Menaquinone-7 Production by Bacillus Subtilis Natto: Fed-batch Glycerol Addition
Menaquinone-7 was produced by fermentation mainly using Bacillus subtilis species. Due to the low concentration of menaquinone-7 in fermentation processes and the associated expense of recovering the menaquinone-7, it was desirable to improve the fermentation concentration. The aim of this study was to determine the effect of fed-batch glycerol addition in the production of menaquinone-7 during...
متن کاملVitamin K-containing dietary supplements: comparison of synthetic vitamin K1 and natto-derived menaquinone-7.
Vitamin K is a cofactor in the production of blood coagulation factors (in the liver), osteocalcin (in bone), and matrix Gla protein (cartilage and vessel wall). Accumulating evidence suggests that for optimal bone and vascular health, relatively high intakes of vitamin K are required. The synthetic short-chain vitamin K(1) is commonly used in food supplements, but recently the natural long-cha...
متن کاملConvergent Synthesis of Menaquinone‐7 (MK-7)
A practical synthesis of menaquinone-7 (MK-7, vitamin K2) in the all-trans form was designed. Stereoselective synthesis of MK-7 was achieved through a “1 + 6” convergent strategy by condensation of two building blocks, menadione monoprenyl derivative (fragment “1”) with hexaprenyl bromide (fragment “6”, 82%). Pd-catalyzed desulfonation with LiEt3BH (78%) was followed by oxidation of the hydroqu...
متن کاملHEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Vitamin K–containing dietary supplements: comparison of synthetic vitamin K1 and natto-derived menaquinone-7
Vitamin K is a cofactor in the production of blood coagulation factors (in the liver), osteocalcin (in bone), and matrix Gla protein (cartilage and vessel wall). Accumulating evidence suggests that for optimal bone and vascular health, relatively high intakes of vitamin K are required. The synthetic short-chain vitamin K1 is commonly used in food supplements, but recently the natural long-chain...
متن کاملLate-onset anaphylaxis after ingestion of Bacillus Subtilis-fermented soybeans (Natto): clinical review of 7 patients.
BACKGROUND Allergic reactions after ingestion of fermented soybeans have rarely been reported. Fermented soybeans were recently reported to be a causative food of IgE-mediated, late-onset anaphylaxis without early phase responses. The objectives of our study are to clarify the clinical and laboratory features and to characterize the allergens in allergy due to fermented soybeans. METHODS Seve...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioresources and Bioprocessing
سال: 2022
ISSN: ['2197-4365']
DOI: https://doi.org/10.1186/s40643-022-00609-0